@article {48, title = {Comparison of Short-Wavelength Reduced-Illuminance and Conventional Autofluorescence Imaging in Stargardt Macular Dystrophy.}, journal = {Am J Ophthalmol}, volume = {168}, year = {2016}, month = {2016 Aug}, pages = {269-278}, abstract = {

PURPOSE: To compare grading results between short-wavelength reduced-illuminance and conventional autofluorescence imaging in Stargardt macular dystrophy.

DESIGN: Reliability study.

METHODS: setting: Moorfields Eye Hospital, London (United Kingdom).

PATIENTS: Eighteen patients (18 eyes) with Stargardt macular dystrophy.

OBSERVATION PROCEDURES: A series of 3 fundus autofluorescence images using 3 different acquisition parameters on a custom-patched device were obtained: (1) 25\% laser power and total sensitivity 87; (2) 25\% laser power and freely adjusted sensitivity; and (3) 100\% laser power and freely adjusted total sensitivity (conventional). The total area of 2 hypoautofluorescent lesion types (definitely decreased autofluorescence and poorly demarcated questionably decreased autofluorescence) was measured.

MAIN OUTCOME MEASURES: Agreement in grading between the 3 imaging methods was assessed by kappa coefficients (κ) and intraclass correlation coefficients.

RESULTS: The mean {\textpm} standard deviation area for images acquired with 25\% laser power and freely adjusted total sensitivity was 2.04 {\textpm} 1.87~mm(2) for definitely decreased autofluorescence (n~= 15) and 1.86 {\textpm} 2.14~mm(2) for poorly demarcated questionably decreased autofluorescence (n~= 12). The intraclass correlation coefficient (95\% confidence interval) was 0.964 (0.929, 0.999) for definitely decreased autofluorescence and 0.268 (0.000, 0.730) for poorly demarcated questionably decreased autofluorescence.

CONCLUSIONS: Short-wavelength reduced-illuminance and conventional fundus autofluorescence imaging showed good concordance in assessing areas of definitely decreased autofluorescence. However, there was significantly higher variability between imaging modalities for assessing areas~of poorly demarcated questionably decreased autofluorescence.

}, keywords = {Adult, Female, Fluorescein Angiography, Fundus Oculi, Humans, Macula Lutea, Macular Degeneration, Male, Middle Aged, Ophthalmoscopy, Optical Imaging, Reproducibility of Results, Stargardt Disease}, issn = {1879-1891}, doi = {10.1016/j.ajo.2016.06.003}, author = {Strauss, Rupert W and Mu{\~n}oz, Beatriz and Jha, Anamika and Ho, Alexander and Cideciyan, Artur V and Kasilian, Melissa L and Wolfson, Yulia and Sadda, SriniVas and West, Sheila and Scholl, Hendrik P N and Michaelides, Michel} }